CHARLES H. CLAPP

Department of Chemistry
216 Rooke Chemistry Building
Bucknell University
Lewisburg, PA 17837

Telephone: (570) 577-3137
Fax: 570-577-1739
E-mail: cclapp@bucknell.edu

Courses taught at Bucknell:

Research

Most of the work in my lab concerns soybean lipoxygenase, a non-heme iron protein that catalyzes the oxygenation of linoleic acid (1) to 13-hydroperoxy-9,11-octadecadienoic acid (2).

The goals of this work are to understand the catalytic mechanism of this enzyme and to discover new inhibitors of the enzyme. It is hoped that the insight derived from this work will contribute to the design inhibitors of human lipoxygenases that are thought to be involved in inflammation, asthma, and possibly atherosclerosis. Studying the soybean enzyme has the advantage that the three-dimensional structure has been determined by X-ray diffraction.

Student projects involve (1) design, synthesis and testing of new inhibitors, (2) synthesis of novel substrates of the enzyme, (3) characterization of the products from novel lipoxygenase substrates, (4) kinetic studies using both spectroscopic and HPLC methods, and (5) studies on the use of site-directed mutagenesis to test hypotheses about substrate binding and catalytic mechanism.

Recent Publications:


Updated: 3/3/13
Creator: J. Clapp