- Professor of Chemistry
Biology / Biochemistry program
- A.B., Bowdoin College
- Ph.D., Harvard University
- Postdoctoral Fellow, Brandeis
- Biochemistry. Mechanisms of
synthesis and mechanistic characterization of enzyme inhibitors.
taught at Bucknell:
- Biochemistry I
- Biochemical Methods
- Organic Chemistry Lab
- Seminar on “Biochemistry of
- Seminar on “Mechanisms of
Pharmaceutically Interesting Enzymes”
- Seminar on “Biotechnology”
- Chemistry, Health, and Disease
- Undergraduate and Graduate
Most of the work in my lab concerns soybean
lipoxygenase, a non-heme iron protein that catalyzes the oxygenation of
linoleic acid (1) to 13-hydroperoxy-9,11-octadecadienoic acid (2).
The goals of this work are to understand the
catalytic mechanism of this enzyme and to discover new inhibitors of the
enzyme. It is hoped that the insight derived from this work will contribute to
the design inhibitors of human lipoxygenases that are thought to be involved in
inflammation, asthma, and possibly atherosclerosis. Because it is highly
stable, the soybean enzyme is well suited for mechanistic studies.
Student projects involve (1) design,
synthesis and testing of new inhibitors, (2) synthesis of novel substrates of
the enzyme, (3) characterization of the products from novel lipoxygenase
substrates, (4) kinetic studies using both spectroscopic and HPLC methods, and
(5) studies on the use of site-directed mutagenesis to test hypotheses about
substrate binding and catalytic mechanism.
- “N-linoleoylamino acids as
chiral probes of substrate binding by soybean lipoxygenase-1,” Clapp, C.
H., Pachuski, J., Bassett, N. F., Bishop, K. A., Carter, G., Young, M.,
Young, T., Fu, Y. Bioorganic Chemistry,
2018, 78, 170-177.
- “Cationic substrates of soybean
lipoxygenase-1,” Chohany; L. E.; Bishop, K. A.; Camic, H.; Sup, S. J.;
Findeis, P. M.; Clapp, C. H. Bioorganic
Chemistry, 2011; 39,
- “Oxygenation of Monounsaturated
Fatty Acids by Soybean Lipoxygenase-1: Evidence for Transient
Hydroperoxide Formation ” Clapp, C. H.; Strulson, M.; Rodriguez, P. C.;
Lo, R.; Novak, M. J. Biochemistry, 2006; 45, 15884-15892.
- “Irreversible Inactivation of
Soybean Lipoxygenase-1 by Hydrophobic Thiols,” Clapp, C. H.; Grandizio, A.
M.; Yang, Y.; Kagey, M.; Turner, D.; Bicker, A; Muskardin, D. Biochemistry,
2002, 41, 11504-11511.
- “Soybean Lipoxygenase-Mediated
Oxygenation of Monounsaturated Fatty Acids to Enones,” Clapp, C. H.;
Senchak, S.; Stover, T. J.; Potter, T. C.; Findeis, P. M.; Novak, M. J. Journal
of the American Chemical Society, 2001, 123, 747-748.
- “The Action of Soybean
Lipoxygenase-1 on 12-Iodo-cis-9-octadecenoic Acid: The Importance of
C(11)-H Bond Breaking,” Clapp, C. H.; McKown, J.; Xu, H.; Grandizio, A.
M.; Yang, G.; Fayer, J. Biochemistry, 2000, 39, 2603-2611.
Creator: J. Clapp