Marie C. Pizzorno-Simpson
Director Cell Biology/biochemistry program
Biology Building Lab-2??
Lab Phone: 609-577-1780
Pizzorno Lab Web page
Faculty Assistant Information:
Karen Shrawder 570-577-1124
B.A., Whittier College
Ph.D., Johns Hopkins School of Medicine
I joined the department in 1996 and was awarded tenure in 2001.
Before arriving at Bucknell I was a visiting professor in the
Biology Department at Vassar College and completed postdoctoral
training in the laboratory of Tom Shenk at Princeton University.
I have been actively studying the molecular and cellular biology
of cytomegalovirus, a human herpesvirus, since 1985. At Bucknell,
I teach BIOL205 - Introduction to Molecules and Cells, BIOL 347-Virology,
BIOL 327-Molecular Biology and a section of BIOL 340-Biochemical
- The main focus of my research has been on the molecular and
cellular biology of eukaryotic viruses, specifically herpesviruses.
Cytomegalovirus (CMV) is a herpesvirus which is very species
and cell-type specific, preferring to grow in differentiated
cells. Work that I started as a post-doctoral fellow suggested
that one of the blocks to infection by CMV in many cell types
may be during entry and uncoating of the virus particle in the
host cell. Students in my lab are involved in using several
experimental approaches to study this problem. These include
localizing viral structural proteins and DNA during early events
of infection using immunofluorescence and in-situ hybridization,
studying protein-protein interactions between viral and cellular
proteins by expressing cloned genes in bacteria, and identifying
biochemical differences between permissive and non-permissive
cells using protein purification. In addition, students learn
how to manipulate cells and virus in tissue culture and basic
recombinant DNA and protein biochemistry techniques. Studying
events that occur early during infection in several cell types
will elucidate both the virus-cell interactions that control
permissivity in CMV and the molecular details of virus entry.
Selected Publications and Meeting Presentations
Pizzorno, M.C., Rice, T.B., Sutton, S. Proteolytic Degradation
of the Tegument Protein pp150 Occurs During Penetration of Cells
by Human Cytomegalovirus. (in preparation)
Ling, E.D., Shutsky, L.. Hart, L., and Pizzorno, M.C. Localization
of the Human Cytomegalovirus Tegument Protein pp150 When Fused
to Green Fluorescent Protein. Arch. Virol. (submitted July 2001)
Shubert, M.A., Pizzorno, M.C., and Nyquist S.E. (2001). Caveolin
and Flotillin Content of Rat Testis Peritubular Myoid Cells and
Detergent-Insoluble Glycosphingolipid-Enriched Membrane Fractions
(DIGS) From These Cells, p 195-201. In B. Robaire, H. Chemes,
and C.R. Morales (eds.), Andrology in the 21st Century, Proceedings
of the VIIth International Congress of Andrology.
Pizzorno, M.C. (2001). Nuclear Cathepsin B-Like Protease Cleaves
Transcription Factor YY1 in Differentiated Cells. Biochim. et
Biophys. Acta 1536:31-42.
Y.-N Chang, K.-T. Jeang, C.-J. Chiou, Y.-J. Chan, M.C. Pizzorno
and G. S. Hayward. 1993. Identification of a Large Bent DNA Domain
and Binding Sites for Serum Response Factor Adjacent to the NFI-Repeat
Cluster and Enhancer Region in the Major IE94 Promoter from Simian
Cytomegalovirus. J. Virol. 67:516-529.