Marie C. Pizzorno-Simpson
Director Cell Biology/biochemistry program
Pizzorno Lab Web page
Biology Building Lab-2??
Lab Phone: 609-577-1780
Faculty Assistant Information:
Karen Shrawder 570-577-1124
B.A., Whittier College
Ph.D., Johns Hopkins School of Medicine
I joined the department in 1996 after completing a visiting
professorship in Biology Department at Vassar College. I received
postdoctoral training at Princeton University, and have been actively
studying the molecular biology of herpesviruses since 1985. At
Bucknell, I teach BIOL205 - Introduction to Molecules and Cells,
Virology BIOL 347, Molecular Biology BIOL 327, and a section of
Biochemical Methods BIOL 340.
The main focus of my research has been on the molecular and
cellular biology of eukaryotic viruses, specifically herpesviruses.
Cytomegalovirus (CMV) is a herpesvirus which is very species and
cell-type specific, preferring to grow in differentiated cells.
Work that I started as a post-doctoral fellow suggested that one
of the blocks to infection by CMV in many cell types may be during
entry and uncoating of the virus particle in the host cell. Students
in my lab are involved in using several experimental approaches
to study this problem. These include localizing viral structural
proteins and DNA during early events of infection using immunofluorescence
and in-situ hybridization, studying protein-protein interactions
between viral and cellular proteins by expressing cloned genes
in bacteria, and identifying biochemical differences between permissive
and non-permissive cells using protein purification. In addition,
students learn how to manipulate cells and virus in tissue culture
and basic recombinant DNA and protein biochemistry techniques.
Studying events that occur early during infection in several cell
types will elucidate both the virus-cell interactions that control
permissivity in CMV and the molecular details of virus entry.
Pizzorno, M.C., Rice, T.B., Sutton, S. Proteolytic Degradation
of the Tegument Protein pp150 Occurs During Penetration of Cells
by Human Cytomegalovirus. (in preparation)
Ling, E.D., Shutsky, L.. Hart, L., and Pizzorno, M.C. Localization
of the Human Cytomegalovirus Tegument Protein pp150 When Fused
to Green Fluorescent Protein. Arch. Virol. (submitted July 2001)
Shubert, M.A., Pizzorno, M.C., and Nyquist S.E. (2001). Caveolin
and Flotillin Content of Rat Testis Peritubular Myoid Cells and
Detergent-Insoluble Glycosphingolipid-Enriched Membrane Fractions
(DIGS) From These Cells, p 195-201. In B. Robaire, H. Chemes,
and C.R. Morales (eds.), Andrology in the 21st Century, Proceedings
of the VIIth International Congress of Andrology.
Pizzorno, M.C. (2001). Nuclear Cathepsin B-Like Protease Cleaves
Transcription Factor YY1 in Differentiated Cells. Biochim. et
Biophys. Acta 1536:31-42.
Y.-N Chang, K.-T. Jeang, C.-J. Chiou, Y.-J. Chan, M.C. Pizzorno
and G. S. Hayward. 1993. Identification of a Large Bent DNA Domain
and Binding Sites for Serum Response Factor Adjacent to the NFI-Repeat
Cluster and Enhancer Region in the Major IE94 Promoter from Simian
Cytomegalovirus. J. Virol. 67:516-529.